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中华猕猴桃(Actinidia chinensis planch)的组织培养技术,1962年英国的穆勒希奇作过研究,1978～1981年中国科学院植物研究所桂跃林等和中国农科院郑州果树研究所黄贞光等也都先后从事这项研究工作,他们都采用MS+玉米素(1 ppm)培养基,从猕猴桃茎段诱导不定芽后,再用MS+IBA(1 PPm)诱导不定芽生根,形成完整的试管植株。1981年,我们在前人研究的基础上,进行了培养基改进试验,采用N_6+6-BA(6ppm)+IBA(1 ppm),不加入玉米素,也能从猕猴桃茎片培养出愈伤组织,并且分化出不定芽进而再诱株生根,形成试管植株并移出盆栽。同时,在含有IBA的培养基中加入低浓度的BA,其诱导发根的效果比仅含一定浓度的IBA,不加BA的培养基更好。
Actinidia chinensis planch tissue culture technology, 1962, the United Kingdom Muleshiqi made a study from 1978 to 1981 Institute of Botany, Chinese Academy of Sciences, such as Guiyue Lin and the Chinese Academy of Agricultural Sciences Zhengzhou Institute of Fruit Huang Zhenguang also In this research, they all used MS + zeatin (1 ppm) medium to induce adventitious buds from kiwifruit stems and then induced shoots of adventitious buds with MS + IBA (1 PPm) to form a complete test tube plant. In 1981, on the basis of previous studies, we conducted medium improvement experiments using N6 + 6-BA (6 ppm) + IBA (1 ppm) without the addition of zeatin and also callus from kiwifruit stems Tissues were formed and adventitious buds were differentiated and rooted again to form test tube plants and removed from pots. At the same time, low concentration of BA was added to IBA-containing medium, which induced hair root more effectively than BA medium containing only a certain concentration of IBA.