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目的研究云南代表性产地8个滇龙胆居群的r DNA ITS遗传差异,评价ITS用于滇龙胆产地鉴别的可行性。方法运用常规PCR法扩增r DNA ITS后直接测序。结果得到8个居群107个样品r DNA中的ITS和5.8Sr DNA全长序列。其中,ITS2在滇龙胆种内居群间和居群内变异最为丰富,但未找到能反映特定居群的稀有单倍型。结论不同产地滇龙胆r DNA-ITS变异较大,但ITS在鉴别滇龙胆的产地上应用价值有限。
Objective To study the r DNA ITS genetic diversity of eight populations of Gentiana yunnanensis in Yunnan, and to evaluate the feasibility of ITS for the identification of Gentiana yunnanensis. Methods The rDNA ITS was amplified by conventional PCR and sequenced directly. Results The ITS and 5.8Sr DNA sequences of 107 samples of 8 populations were obtained. Among them, ITS2 had the most abundant variation among populations and populations within Gentiana yunnanensis, but rare haplotypes that could not reflect specific populations were found. Conclusion The variation of DNA-ITS in Gentiana yunnanensis from different habitats is larger, but its value in identifying the origin of Gentiana yunnanensis is limited.