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目的建立混凝沉淀富集回收水中隐孢子虫卵囊的方法。方法以4μm荧光微球为研究对象,采用混凝沉淀试验,通过正交设计筛选出最佳混凝剂[聚合氯化铝(PAC)、水溶性壳聚糖(WSC)、1 mol/L的NaOH溶液用量]的配方,并观察不同pH值(6～9)、浑浊度(0～15 NTU)和水温(5～15℃)对回收率的影响。结果确定了最佳混凝配方,聚合氯化铝浓度为80 mg/L,壳聚糖浓度为30 mg/L,1 mol/L氢氧化钠溶液用量为0.6 ml。当pH值在6～9范围内,回收率均在86%以上;当水温在5～25℃范围内,回收率在55%以上;当浑浊度为0～15 NTU范围内,回收率均在90%以上。且回收率均随着pH值、水温和浑浊度的升高而上升。向实际水样投加隐孢子虫,采用混凝沉淀法与EPA1623法进行富集比较,差异无统计学意义。结论该方法能够高效富集回收水中的隐孢子虫卵囊,并对实验条件要求较低,适用于一般实验室对水中隐孢子虫卵囊的检测。
Objective To establish a method for coagulation sedimentation enrichment of Cryptosporidium oocysts in water. Methods A 4 μm fluorescent microsphere was used as the research object. The optimal coagulant (PAC, water soluble chitosan (WSC), 1 mol / L NaOH solution]. The effects of different pH value (6 ~ 9), turbidity (0 ~ 15 NTU) and water temperature (5 ~ 15 ℃) on the recovery were observed. The results showed that the best coagulation formula was obtained. The concentration of PAC was 80 mg / L, the concentration of chitosan was 30 mg / L and the amount of 1 mol / L sodium hydroxide solution was 0.6 ml. When the pH value ranged from 6 to 9, the recoveries were above 86%. When the water temperature was in the range of 5 ~ 25 ℃, the recovery was over 55%. When the turbidity was 0 ~ 15 NTU, the recoveries were both within More than 90. The recoveries increased with the increase of pH value, water temperature and turbidity. Cryptosporidium was added to the actual water sample, and the enrichment by coagulation-sedimentation method and EPA1623 method showed no significant difference. Conclusion The method can efficiently collect Cryptosporidium oocysts in water and has low requirements on experimental conditions. It is suitable for the detection of Cryptosporidium oocysts in water in general laboratories.