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目的研究镉与汞单独及联合染毒对人胚肝细胞(L02细胞)凋亡及DNA损伤的作用。方法以0.01～100μmol/L的氯化镉、氯化汞以及等浓度的氯化镉、氯化汞混合液对L02细胞染毒24 h,采用MTT法测定细胞的存活率,根据Finney法判断镉与汞对L02细胞的联合作用类型;采用单细胞凝胶电泳技术(SCGE)和流式细胞术(FCM)检测L02细胞的DNA损伤和细胞凋亡情况。结果 0.01μmol/L的氯化镉、氯化汞单独及联合染毒可以刺激细胞的生长,但≥1μmol/L的氯化镉、氯化汞单独及联合染毒可显著抑制细胞的生长;且联合作用表现为相加作用。镉、汞单独及联合作用可致L02细胞的DNA损伤率和细胞凋亡率均显著高于对照组;且随着染毒浓度的升高,L02细胞的DNA损伤率和细胞凋亡率均呈上升趋势。结论镉汞联合染毒引起的DNA损伤和细胞凋亡可能存在一定的协同效应,这可能是由于镉、汞诱导细胞发生氧化应激所致。
Objective To study the effects of cadmium and mercury alone and in combination on the apoptosis and DNA damage of human embryonic hepatocytes (L02 cells). Methods L02 cells were exposed to 0.01 ~ 100μmol / L cadmium chloride, mercuric chloride, and equal concentrations of cadmium chloride and mercuric chloride for 24 hours. The survival rate of the cells was determined by MTT method. Cadmium was determined by Finney method And mercury on L02 cells. The DNA damage and apoptosis of L02 cells were detected by single cell gel electrophoresis (SCGE) and flow cytometry (FCM). Results 0.01μmol / L cadmium chloride and mercuric chloride alone or in combination could stimulate the growth of cells. However, the addition of 1μmol / L cadmium chloride and mercuric chloride alone and in combination could significantly inhibit the growth of cells. The combined effect is additive. The DNA damage rate and apoptosis rate of L02 cells were significantly higher than that of the control group both alone and in combination with cadmium and mercury. The DNA damage rate and apoptosis rate of L02 cells were increased with the increase of exposure concentration Upward trend. Conclusion There may be a synergistic effect between DNA damage and apoptosis induced by combined exposure to cadmium and mercury, which may be due to the oxidative stress induced by cadmium and mercury.