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目的:周皮细胞的分化在血管新生过程中具有重要作用,没有周皮细胞及其分泌组建的基底膜的支撑,毛细血管就没有正常的功能。作者以前的工作证明周皮细胞可能来源于外周血循环纤维细胞(PBFC),但血管内皮细胞如何趋化PBFC还不清楚。本实验重点观察CXCL8及其受体CXCR2在血管内皮细胞趋化PBFC中的作用。方法:分离纯化人PBFC后与人微血管内皮细胞(HDMEC)共培养,观察共培养条件下PBFC的形态学改变,并检测PBFC细胞内CXCR2 mRNA表达和HDMEC内CXCL8 mRNA的表达。结果:与HDMEC共培养后,PBFC由梭形向菱形改变;HDMEC内的CXCL8 mRNA水平与PBFC共培养24小时后增高约10倍,培养后48小时仍维持在高水平;PBFC内的CXCR2 mRNA水平在共培养后24小时增高约3倍,且在培养后24小时仍维持在较高水平。结论:CXCL8/CXCR2可能参与了血管内皮细胞趋化PBFC的过程。
OBJECTIVE: The differentiation of pericytes plays an important role in angiogenesis. Without the support of pericytes and the basement membrane secreted by them, capillaries have no normal function. Previous work by the authors demonstrated that peripheral blood cells may originate from peripheral blood circulating fibroblasts (PBFCs), but how endothelial cells become chemotactic to PBFCs remains unclear. This experiment focused on the CXCL8 and its receptor CXCR2 in vascular endothelial cell chemotaxis PBFC role. Methods: The human PBMCs were isolated and purified and co-cultured with human microvascular endothelial cells (HDMEC). The morphological changes of PBFC were observed under co-culture conditions. The expression of CXCR2 mRNA and CXCL8 mRNA in HDFC were detected. Results: After coculture with HDMEC, the expression of PBFC changed from fusiform to rhombus. The level of CXCL8 mRNA in HDMEC increased about 10-fold after co-cultured with PBFC for 24 hours and maintained at a high level 48 hours after culture. CXCR2 mRNA level in PBFC Increased about 3-fold at 24 hours after co-culture and remained at a high level 24 hours after the culture. Conclusion: CXCL8 / CXCR2 may be involved in the chemotaxis process of PBMC to vascular endothelial cells.