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本研究以慢性B淋巴细胞性白血病患者血清IgM免疫BALB／C／J‘鼠，取其脾细胞，采用细胞融合技术建立分泌抗慢性淋巴细胞白血病（BCLL）同种型和独特型单克隆抗体的杂交瘤细胞株。采用一系列免疫化学，细胞免疫学等技术对10株独特型单抗和5株同种型单抗的特异性，亲和力，识别抗
In this study, BALB / c / J ’mice were immunized with serum IgM from patients with chronic B lymphocytic leukemia and their spleen cells were obtained. Cell fusion was used to establish a monoclonal antibody that secretes anti-CDLL isotypes and idiotypic monoclonal antibodies Hybridoma cell line. Using a series of immunochemistry, cell immunology and other technologies on 10 unique monoclonal antibody and 5 isotype monoclonal antibody specificity, affinity,