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本研究以湘豆3号大豆种子为外植体探究消毒条件与消毒效果间关系,以胚尖为接种材料,建立了湘豆3号胚尖再生体系,以卡那霉素为筛选剂,获得抗生素选择最适浓度。研究表明:70%的酒精消毒1 min后配合5%的Na Cl O消毒10 min为湘豆3号大豆种子最佳的消毒方法;在MS+0.1 mg/L TDZ的培养基中胚尖丛生芽诱导率最高,达到96.7%;不定芽在不添加生长素的1/2 MS培养基中能正常生根,生根率达到76.67%;在MS培养基中添加浓度为75~100 mg/L的卡那霉素为最适宜湘豆3号抗生素选择压力。
In this study, Xiang bean No. 3 soybean seeds as explants to explore the relationship between disinfection conditions and disinfection effect, the embryo as the inoculum material, the establishment of a Xianghu 3 embryo regeneration system with kanamycin as a screening agent, obtained Antibiotics choose the optimal concentration. The results showed that disinfection of 70% alcohol for 1 min followed by disinfection with 5% Na Cl O for 10 min was the best method for sterilizing soybean seed of Xiang Dou No.3. In the MS + 0.1 mg / L TDZ medium, The highest induction rate reached 96.7%. Adventitious buds could root normally in 1/2 MS medium supplemented with auxin and the rate of rooting reached 76.67%. The MS medium supplemented with 75-100 mg / L Kana As the best choice for the choice of antibiotics,