论文部分内容阅读
多毛番茄(Solanum habrochaites)为重要的番茄种质资源,其叶表存在大量次生代谢物质,对多种虫害具有趋避或/和毒害作用。利用气相色谱-质谱联用(GC-MS)技术分析栽培番茄(S.lycopersicum)9706与3份多毛番茄(LA2329、LA1777和PI134417)材料叶表次生代谢物质。结果表明,3份多毛番茄叶表可检测到的次生代谢物质种类和总含量均高于普通番茄,同时多毛番茄亚种间次生代谢物质的种类和含量也存在差异。普通番茄叶表次生代谢物质为3种单萜和3种倍半萜类物质,其中单萜和倍半萜类物质分别占次生代谢物质总量的60.3%和39.7%。多毛番茄LA2329和LA1777叶表倍半萜类物质的种类和含量较高,有些萜类物质具有物种特异性。如LA2329中含量最高的α-姜烯,其含量为2 409.1μg·g–1;LA1777中含量较高的γ-榄香烯和E-β-法尼烯,含量分别为573.3μg·g–1和289.9μg·g–1,在其它番茄材料中未检测到这3种倍半萜类物质。PI134417中含量最高的是月桂酸乙酯,其含量为5 312.8μg·g–1,在普通番茄中这一物质未见报道。PI134417中甲基酮类物质含量也较高,其中2-十一烷酮和2-十三烷酮的含量分别为689.8μg·g–1和1 459.7μg·g–1。研究结果可为番茄种质资源利用和次生代谢物质开发提供理论依据。
Solanum habrochaites are important tomato germplasm resources. There are a lot of secondary metabolites in the leaf surface of the solanum habrochaites, which can evade or / and poison many kinds of pests. The secondary metabolites in leaves of cultivated tomato (S. lycopersicum) 9706 and three copies of hairy tomato (LA2329, LA1777 and PI134417) were analyzed by gas chromatography-mass spectrometry (GC-MS). The results showed that the content of secondary metabolites detected in the leaves of three hirsutum cultivars was higher than that of common tomatoes, and there were also differences in the types and contents of secondary metabolites among the three hirsute. Three kinds of monoterpenes and three kinds of sesquiterpenes were found in the common tomato leaf surface secondary metabolites. Monoterpene and sesquiterpene accounted for 60.3% and 39.7% of the total secondary metabolites respectively. Hirsute tomato LA2329 and LA1777 leaf table sesquiterpenoids species and content is high, some terpenoids species-specific. For example, the highest content of α-gingerone in LA2329 was 2 409.1 μg · g-1, the content of γ-elemene and E-β-farnesene in LA1777 were 573.3 μg · g- 1 and 289.9μg · g-1, respectively. No other sesquiterpenoids were detected in other tomato materials. PI134417 is the highest content of ethyl laurate, its content of 5 312.8μg · g-1, in the ordinary tomato this material has not been reported. The content of methyl ketone in PI134417 was also higher, and the contents of 2-undecanone and 2-tridecanone were 689.8μg · g-1 and 1 459.7μg · g-1, respectively. The results provide a theoretical basis for the utilization of tomato germplasm resources and the development of secondary metabolites.