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目的:研究核苷酸结合寡聚化结构域(nucleotide-binding oligomerization domain,NOD)相关蛋白在烟曲霉(Af)感染小鼠肺组织中的表达。方法:通过经鼻气道滴人Af孢子的方法建立小鼠感染Af的模型,对照组小鼠经鼻气道内滴人等量的生理盐水。分别在不同时间点观察肺部Af负荷及病理学改变,采用RT-PCR方法检测各时间点的NOD1、NOD2及受体相互作用蛋白2(receptor- interacting protein 2,RIP2)mRNA在肺组织中的表达。结果:1)感染组小鼠肺部Af负荷24h达最高峰,48h迅速下降。2)两组小鼠均表现为支气管周围炎症为主的病理改变,感染组小鼠24 h炎症细胞浸润最显著,48 h炎症细胞浸润减轻。对照组小鼠病理损害明显轻于感染组。3)NOD1、NOD2、RIP2 mRNA表达在接种Af后12 h、24 h、48 h显著升高(P<0．05)。对照组各时间点NOD1、 NOD2、RIP2 mRNA表达无显著差异。结论:NOD蛋白可能为Af的胞浆内模式识别受体(pattern。recognition receptors,PRRs),在抗 Af肺部感染的免疫反应中发挥一定作用。
OBJECTIVE: To study the expression of nucleotide-binding oligomerization domain (NOD) -related protein in the lung tissue of mice infected with Aspergillus fumigatus (Af). Methods: The model of Af infection in mice was established by intranasal instillation of Af spores. In the control group, the same amount of normal saline was administered to the mice via nasal airway. The lungs were exposed to Af load and pathological changes at different time points respectively. The expression of NOD1, NOD2 and receptor-interacting protein 2 (RIP2) mRNA in lung tissue were detected by RT-PCR at different time points expression. Results: 1) In the infected group, the lungs of Af mice reached the peak at 24 hours and decreased rapidly at 48 hours. 2) The two groups of mice showed bronchial inflammation mainly pathological changes, the infected mice 24 h inflammatory cell infiltration was the most significant, 48 h inflammatory cell infiltration reduced. The mice in the control group were significantly less pathologically damaged than those in the infected group. 3) NOD1, NOD2 and RIP2 mRNA expression increased significantly at 12 h, 24 h and 48 h after Af vaccination (P <0.05). There was no significant difference in NOD1, NOD2 and RIP2 mRNA expression in control group at all time points. CONCLUSIONS: The NOD protein may be the intracytoplasmic pattern recognition receptor (PRRs) of Af, which plays a role in the immune response against Af lung infection.