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构建组织工程化软骨常常需要体外扩增软骨细胞。骨但是,软骨细胞在体外培养、扩增过程中常发生去分化((dedifferentiation)而丧失细胞表型~([1]),导致再生软骨中1II型胶原、氨基葡萄糖(GAG)合成减少,易发生退变。区目前有关去分化的具体机理尚未阐明,但以往的研究表短明去分化主要与体外扩增的软骨细胞缺乏细胞与细胞外结基质(extracellular matrix,ECM)之间有效的信号刺激有高关~([2-3])。Integrin(整合素)作为细胞膜上的一种跨膜糖蛋半
The construction of tissue-engineered cartilage often requires the in vitro expansion of chondrocytes. However, chondrocytes are cultured in vitro and dedifferentiation often results in the loss of cell phenotype ~ ([1]), leading to a decrease in the synthesis of type 1 collagen II and glucosamine (GAG) in regenerated cartilage, which is prone to occur Degeneration The current mechanisms for dedifferentiation have not been elucidated, but previous studies have shown that dedifferentiation is mainly due to the lack of effective signal stimulation between chondrocytes expanded in vitro and extracellular matrix (ECM) There are high-off ~ ([2-3]). Integrin (Integrin) as a cell membrane transmembrane glycoprotein half