【摘 要】
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OBJECTIVE:To elucidate the protective effects of Renshen (Radix Ginseng) and Fuzi (Radix Aconiti Lateralis Preparata) on myocardial infarction (MI)through regulating myocardial autophagy.METHODS:Thirty-one male Sprague-Dawley rats were randomized into fiv
【机 构】
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Pharmacology and Toxicology Laboratory,Bei-jing Institution of Radiation Medicine,Beijing 100850,Chi
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OBJECTIVE:To elucidate the protective effects of Renshen (Radix Ginseng) and Fuzi (Radix Aconiti Lateralis Preparata) on myocardial infarction (MI)through regulating myocardial autophagy.METHODS:Thirty-one male Sprague-Dawley rats were randomized into five groups (n =6 or 7 for each).After treatment for 3 weeks,electrocardio-gram (ECG) and cardiac function were recorded.Hematoxylin and eosin (HE) staining was used to detect pathological changes in the heart.En-zyme-linked immunosorbent assay (ELISA) was used to detect serum B-type brain natriuretic pep-tide (BNP),cardiac troponin T (cTnT),tumor necro-sis factor-α (TNF-α),and serum inflammatory cyto-kines.Metabolomic analysis was used to identify differential biomarkers of MI in rats.Immunohisto-chemistry and western blotting were used to de-tect BNP,cTnT,TNF-α,LC3B,Beclin-1,p62,and ade-nosine monophosphate activated protein kinase(AMPK) expression in cardiac tissue.RESULTS:Fuzi (Radix Aconiti Lateralis Preparata)alone or Renshen (Radix Ginseng) plus Fuzi (Radix Aconiti Lateralis Preparata) markedly ameliorated cardiac dysfunction and abnormal ECGs,demon-strated by decreases in the heart weight/body weight ratio,BNP,and cTnT.Pro-inflammation cyto-kine interleukin (IL)-lα significantly decreased and anti-inflammatory cytokine IL-10 significantly in-creased in Renshen (Radix Ginseng) single or Rensh-en (Radix Ginseng) plus Fuzi (Radix Aconiti Lateralis Preparata) groups compared with the control group.HE results suggested that co-treatment pro-duced a greater reduction in cardiomyocyte cross-sectional area than Renshen (Radix Ginseng)or Fuzi (RadixAconiti Lateralis Preparata) alone.Ren-shen (Radix Ginseng) plus Fuzi (Radix Aconiti Latera-lis Preparata) reversed these changes to different degrees in MI rats.Furthermore,Renshen (Radix Ginseng) plus Fuzi (Radix Aconiti Lateralis Prepara-ta) down-regulated LC3B,Beclin-1,and AMPK ex-pression in cardiac tissue and upregulated p62 expression.CONCLUSIONS:Renshen (Radix Ginseng) plus Fuzi(Radix Aconiti Lateralis Preparata) may have a great-er effect on heart injury induced by MI in rats than Fuzi (Radix Aconiti Lateralis Preparata) treatment alone,and the underlying mechanism may be asso-ciated with the regulation of myocardial autophagy and anti-inflammation effects.These results pro-vide fresh insight into the mechanism of co-treat-ment with Renshen (Radix Ginseng) and Fuzi (Radix Aconiti Lateralis Preparata) for MI.
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