Yishen Huoxue decoction(益肾活血方)attenuates unilateral ureter-ic obstruction-induced renal fibrosis and

• 作 者: ZHONG Jian FANG Guiyu WANG Zixia CHEN Ping LU Dengyong SHI Xiaodong
• 机 构: Department of Nephrology,the First Affiliated Hospital of Guangxi University of traditional Chinese
• 出 处: 中医杂志（英文版）
• 发表日期: 2021年6期
• 关键词: renal fibrosis ureteral obstruction Sir-tuin 3 reactive oxygen species hypoxia Y

OBJECTIVE:To investigate the effeicacy of Yishen Huoxue decoction (YSHX) on renal fibrosis induced by unilateral ureteric obstruction (UUO),and on re-active oxygen species (ROS) homeostasis in human umbilical vein endothelial cells (HUVECs).METHODS:Forty male mice were randomly divid-ed into six groups,sham group,UUO group,UUO+resveratrol (RSV) (15 mg/kg) group,UUO+YSHX 20 mg/kg group (UUO+YSHX-L),UUO + YSHX 40 mg/kg group (UUO + YSHX-M),UUO + YSHX 80 mg/kg group (UUO + YSHX-H).Western blotting was used to measure protein expression levels.Re-verse transcription-quantitative polymerase chain reaction was used to measure the mRNA expres-sion.Immunohistochemistry was used to examine the histopathological changes of kidney tissue sam-ple.Cell apoptosis was measured by Annexin WPI staining.Cell viability was measured using CCK-8/WST-8 assay.RESULTS:YSHX treatment reduced α-SMA and Col-4 expressions,and increased CD31 and VE-cad-herin expressions in UUO model mice.In vitro,YSHX increased cell viability and decreased apoptosis of HUVECs under hypoxic conditions.YSHX inhibited ROS generation by activating adenosine mono-phosphate-activated protein kinase (AMPK)/peroxi-some proliferator-activated receptor coactivator-1α(PGC-1α)/silent mating-type information regulation 2 homolog 3 (Sirt3) signaling.CONCLUSION:YSHX treatment reduced 109KJ UUO-induced renal injury and fibrosis.Further-more,YSHX treatment attenuated hypoxia-induced oxidative stress by regulating AMPK/PGC-1α/Sirt3 signaling.