Pingchuan formula (平喘方) improves allergic asthma in mice through inhibiting nuclear factor-kappa B/m

• 作 者: WU Mingyun YU Jianer BAI Li XUE Zheng JIANG Shenhua LI Liqing PIAO Xiang XU Wanchao WANG Jiani SHEN Qian
• 机 构: Department of Pediatrics,Shanghai Municipal Hospital of Traditional Chinese Medicine,Shanghai 200071
• 出 处: 中医杂志（英文版）
• 发表日期: 2021年6期
• 关键词: asthma NF-kappa B p38 mitogen-acti-vated protein kinases signal transduction Pin

OBJECTIVE:To examine the role and decipher the mechanism of Pingchuan formula(平喘方,PCF)in treating allergic asthma.METHODS:The mice were treated with saline,dexamethasone (DXM) and PCF for 1 week after the asthma model was established and their respi-ratory function including respiratory resistance (RI),pulmonary dynamic compliance (Cdyn) and maxi-mum voluntary ventilation (MW) were measured.In addition,cellular changes in bronchoalveolar la-vage fluid (BALF) and pathological changes in lung biopsy as well as the expression level of α-smooth muscle actin (α-SMA),transforming growth fac-tor-beta1 (TGF-β1)in BALF and interleukin-5 (IL-5),interleukin-13 (IL-13),tumor necrosis factor-α(TNF-α),interferon-y (IFN-y),nuclear factor-kappa B-p65 (NF-κBp65),inhibitor-α of nuclear transcrip-tion factor κB (IκBα),p38 mitogen-activated pro-tein kinase (p38MAPK),c-jun n-terminal kinase(JNK) and its phosphorylated proteins in lung tis-sue were also examined and compared among dif-ferent groups.RESULTS:Our data suggested that the respiratory functions were significantly improved and the path-ological changes ameliorated in the DXM group and the PCF group compared to the model group.Both DXM and PCF effectively decreased the num-ber of eosinophils,lymphocytes,and neutrophils in BAL as well as the secretion ofα-SMA and TGF-β1,IL-5,IL-13,while increased the expression of TNF-α and IFN-y.Furthermore,our study indicated that the NF-κBp65,IκBα,p38MAPK and JNK pathways were inhibited under the treatment of PCF.CONCLUSION:Our data indicated that PCF can at-tenuate the inflammatory response in asthma thr-ough inhibiting the NF-κB/MAPK signaling path-way.This study not only supported the use of PCF in allergic asthma in clinic but also shed light upon a further understanding of the disease pathogenesis.