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棒状体是疟原虫入侵红细胞的细胞器。棒状体相关蛋白(RAP1)由于株间差异小,是疟疾疫苗的重要候选抗原。本文对8种RAP1的重组蛋白的抗原性进行研究。 用PCR将恶性疟原虫K1株的8种RAP1重组蛋白的核酸扩出,装入PGEX载体,然后纯化。所有蛋白于-70℃保存。给一组6—8周断乳雌鼠腹腔注射50μg其中任一蛋白加弗氏佐剂,36天后提取血清。再注射50μg同种蛋白加不完全弗氏佐剂,13,21天后分别提取血清,将上述血清按1:50,
Canes are organelles that plasmodia invades erythrocytes. The rod-associated protein (RAP1) is an important candidate antigen for malaria vaccine due to the small difference between strains. In this paper, the antigenicity of eight RAP1 recombinant proteins was studied. The nucleic acids of the eight RAP1 recombinant proteins of Plasmodium falciparum K1 strain were amplified by PCR, loaded into PGEX vector, and then purified. All proteins are stored at -70 ° C. A group of 6-8 week-old infants were intraperitoneally injected with 50 μg of either protein plus Freund’s adjuvant, and serum was extracted after 36 days. Then inject 50μg of the same protein plus incomplete Freund’s adjuvant, after 13 and 21 days, respectively, extract the serum, the above serum by 1:50,