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采用自组装技术将人工合成的细胞粘附分子精氨酸-甘氨酸-天冬氨酸-半胱氨酸(RGDC)和防止细胞非特异性粘附的三乙二醇单-11-巯基十一烷基醚(TGME)修饰于金电极表面,制备了致密的、稳定的、对细胞具有特异性粘附作用的传感界面。通过调整RGDC与TGME的比例,比较了其对大鼠心肌细胞(H9C2)的特异性粘附效果。以铁氰化钾为探针的实验结果表明,该界面对大鼠心肌细胞有很好的特异粘附作用。此外,还考察了该界面在Cr(Ⅵ)溶液中的电化学行为。结果表明,随着Cr(Ⅵ)浓度的增高氧化还原峰的峰电流增大,而峰电位均负向移动。其电化学参数变化与显微镜下观察到的Cr(Ⅵ)对细胞形态的模拟结果表明,所构建的细胞特异性粘附界面及电化学分析技术可望用于重金属对细胞的毒性研究。
The self-assembly technique was used to synthesize the synthetic cell adhesion molecule arginine-glycine-aspartic acid-cysteine (RGDC) and triethylene glycol mono-11-mercapto undecane The ether (TGME) was modified on the surface of gold electrode to prepare a dense and stable sensing interface with specific cell adhesion. By adjusting the ratio of RGDC to TGME, the specific adhesion to rat cardiomyocytes (H9C2) was compared. Experimental results using potassium ferricyanide as a probe showed that the interface has a good specific adhesion to rat cardiomyocytes. In addition, the electrochemical behavior of this interface in Cr (Ⅵ) solution was also investigated. The results showed that with the increase of Cr (Ⅵ) concentration, the peak current of redox peak increased while the peak potential shifted negatively. The change of electrochemical parameters and the observed Cr (Ⅵ) under the microscope showed that the cell-specific adhesion interface and electrochemical analysis could be used to study the toxicity of heavy metals to cells.