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DNA骨架磷硫酰化修饰是指在天然DNA骨架上发现的P—O键被P—S键替换的化学修饰,属于首例生理修饰,具有磷硫修饰的DNA在Tris缓冲体系中电泳时具有DNA降解表型.研究发现DNA骨架磷硫修饰属于复制后修饰,具有如下三个特征:Rp型立体修饰,序列专一性、分布广泛性.这种修饰由五个基因组成的dnd基因簇(dndA-E)编码的蛋白控制,但这些蛋白具体的作用机制目前还不清楚.为了将来更好地研究DNA磷硫修饰机制,本文综述了DNA磷硫酰化修饰的发现历程,特点,参与DNA磷硫修饰的蛋白结构研究进展,以及磷硫修饰的DNA作为抗氧化剂研究进展.同时,对DNA磷硫修饰机制、生理功能等研究目前所面临的困难,如硫原子如何渗入DNA骨架,参与DNA磷硫修饰的五个蛋白是如何协调作用完成DNA磷硫修饰的机理等难题进行了简单概括,以为相关研究提出一些可能的方向.
DNA Skeleton Phosphoryl Sulfonylation Modification refers to the chemical modification in which the P-O bond found on the natural DNA backbone is replaced by a P-S bond. It belongs to the first physiological modification. Phosphorus sulfur modified DNA has the following DNA degradation phenotype.The study found that the DNA backbone phosphorus and sulfur modifications belong to the post-replication modification, has the following three characteristics: Rp stereospecific, sequence specificity, widely distributed.This modification consists of five genes dnd gene cluster dndA-E), but the specific mechanism of action of these proteins is still unclear.In order to study the mechanism of DNA phosphorothiolate modification better in the future, this paper reviewed the discovery process and characteristics of DNA phosphorothionylation, Phosphorus and sulfur modified protein structure research progress, as well as phosphorus sulfur modified DNA as antioxidant research progress.Meanwhile, the current study of DNA phosphorothioate modification mechanism, physiological function, such as how sulfur atoms penetrate the DNA backbone, participate in DNA Phosphorus sulfur modified five proteins is how to coordinate the completion of the mechanism of DNA phosphorothioate and other problems are briefly summarized in order to make some possible directions for the relevant research.