论文部分内容阅读
目的观察实验性兔VX2脑瘤的灌注CT改变,并与病理及免疫组织化学(简称免疫组化)结果对照,检验灌注CT反映脑瘤血管生成的效能。方法20只VX2脑瘤荷瘤兔按随机数字表分成3周以下组和3周以上组行灌注CT检查,测量肿瘤、瘤周及对侧正常脑组织的血容量(blood volume,BV),血流量(bloodflow,BF)和表面通透性(permeabilitysurface,PS)。大体标本观察肿瘤伊文思蓝染色级别。免疫组化观察血管内皮生长因子(vascularendothelialgrowthfactor,VEGF)表达率和微血管密度(microvesseldensity,MVD)。结果肿瘤区BV、BF和PS值[分别为(13.25±4.58)ml·100g-1、(166.14±69.62)ml·100g-1·min-1、8.01ml·min-1·100g-1]均明显高于瘤周[分别为(2.38±0.80)ml·100g-1、(62.49±25.83)ml·100g-1·min-1、0.03ml·min-1·100g-1]及对侧正常脑组织[分别为(2.24±0.75)ml·100g-1、(55.72±21.24)ml·100g-1·min-1、0.04ml·min-1·100g-1]差异有统计学意义(P值均=0.000)。3周以上组肿瘤区BV、BF和MVD[分别为(16.41±4.12)ml·100g-1、(208.77±63.00)ml·100g-1·min-1、(61.20±12.93)个/高倍视野]均明显高于3周以下组[(10.09±2.27)ml·100g-1、(123.51±47.18)ml·100g-1·min-1、(41.40±7.34)个/高倍视野],差异有统计学意义(P值均<0.01)。肿瘤区MVD与BV(r=0.915,P=0.000)和BF(r=0.901,P=0.000)呈显著正相关,与PS(r=0.459,P=0.042)呈正相关;肿瘤蓝染级别与PS(rs=0.861,P=0.000)呈显著正相关。结论灌注CT可明确区分肿瘤与瘤周和正常组织,准确反映肿瘤的血管生成情况,为脑瘤评价提供重要资料。
Objective To observe the changes of perfusion CT in experimental rabbit VX2 brain tumor, and to compare the results of pathology and immunohistochemistry (immunohistochemistry) to evaluate the efficacy of perfusion CT in reflecting the angiogenesis of brain tumor. Methods 20 VX2 brain tumor-bearing rabbits were divided into 3 weeks group and 3 weeks group according to random number table to perform perfusion CT examination. The blood volume (BV), blood Blood flow (BF) and permeability surface (PS). Gross specimen observation Evans blue staining levels. Immunohistochemistry was used to observe the expression of vascular endothelial growth factor (VEGF) and microvessel density (MVD). Results The values of BV, BF and PS in tumor area were (13.25 ± 4.58) ml · 100g-1 and (166.14 ± 69.62) ml · 100g-1 · min-1 and 8.01ml · min-1 · 100g-1 (2.38 ± 0.80) ml · 100g-1, (62.49 ± 25.83) ml · 100g-1 · min-1 and 0.03% · min-1 · 100g-1, respectively) The differences were statistically significant (P values were (2.24 ± 0.75) ml · 100g-1, (55.72 ± 21.24) ml · 100g-1 · min -1,0.04ml · min -1 · 100g -1] = 0.000). The BV, BF and MVD in tumor area of more than 3 weeks were (16.41 ± 4.12) ml · 100g-1, (208.77 ± 63.00) ml · 100g-1 · min-1 and (61.20 ± 12.93) Were significantly higher than those in the group of less than 3 weeks [(10.09 ± 2.27) ml · 100g-1, (123.51 ± 47.18) ml · 100g-1 · min-1 and (41.40 ± 7.34) Significance (P <0.01). MVD was positively correlated with BV (r = 0.915, P = 0.000) and BF (r = 0.901, P = 0.000), and positively correlated with PS (r = 0.459, P = 0.042) (rs = 0.861, P = 0.000) showed a significant positive correlation. Conclusion Perfusion CT can clearly differentiate tumor from peritumoral and normal tissues and accurately reflect tumor angiogenesis, providing important information for evaluating brain tumor.