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目的:检测二肽酶IV(DPP IV)在胃癌组织中的表达,探讨其在胃癌预后中的价值。方法:收集本院手术后的胃癌组织及对应癌旁正常组织112例,用免疫组织化学方法定性比较胃癌组织及癌旁正常胃黏膜组织中DPP IV的表达;Western blot定量比较胃癌组织及癌旁正常组织中DPP IV蛋白的表达。结果:免疫组织化学结果示DPP IV在癌旁正常组织中有3例(占2.7%)表达缺失;胃癌组织中有78例(占69.6%)表达缺失;Western blot结果示DPP IV在胃癌组织中的含量明显低于癌旁正常组织;多因素分析示DPP IV是胃癌不良预后的独立预测因子(P<0.001)。结论:DPP IV表达缺失是胃癌不良预后的独立预测因子。
Objective: To detect the expression of dipeptidyl-IV (DPP IV) in gastric cancer and explore its value in the prognosis of gastric cancer. Methods: A total of 112 gastric cancer tissues and corresponding normal tissues were collected from our hospital. The expression of DPP IV in gastric cancer tissue and adjacent normal gastric mucosa tissues was qualitatively compared by immunohistochemistry. Western blot was used to quantify the expression of DPP IV in gastric cancer tissues and adjacent normal tissues DPP IV protein expression in normal tissues. Results: The results of immunohistochemistry showed that there were 3 cases (2.7%) of the normal tissue adjacent to DPP IV, and the expression of DPP IV was absent in 78 cases (69.6%). Western blot showed that DPP IV in gastric cancer tissues (P <0.001). The multivariate analysis showed that DPP IV was an independent predictor of poor prognosis of gastric cancer (P <0.001). Conclusion: The loss of DPP IV expression is an independent predictor of poor prognosis in gastric cancer.