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为了解体外培养细胞的改变与细胞癌变的关系,管观察了中国地鼠的正常细胞在短期培养过程中和长期培养后的改变,并与多瘤病毒转化的细胞(Polyoma virlls fra-nsformed cell line)进行了此较。 正常细胞在培养两周内,酸性磷酸酶(ACP)琥珀酸脱氢酶(MDH),乳酸脱氢酶(LDH)升高;DNA蛋白质略增;脂类不变;碱性磷酸酶下降。在培养七个月的过程中,细胞形态由成纤维变为上皮,逐渐出现双核、核出芽等核型变化,其后酸性磷酸
To investigate the relationship between cell changes and cell carcinogenesis in vitro, the changes of normal cells in Chinese hamster during short-term culture and after long-term culture were observed and compared with Polyoma virlls fra-nsformed cell line This is done. During the two weeks of culture, normal cells had elevated ACP succinate dehydrogenase (MDH) and lactate dehydrogenase (LDH); DNA protein slightly increased; lipids unchanged; alkaline phosphatase decreased. In the course of seven months of culture, the morphology of the cells changed from fibroblast to epithelium, karyotype changes gradually appeared, such as binuclear and nuclear sprouts. Subsequently, the acid phosphates