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目的：进一步研究蛋白激酶C抑制剂Staurosporine在血小板聚集、蛋白磷酸化、肌动蛋白聚合中的作用。方法：以32P－Na2HPO4标记血小板；以血小板聚集仪测定血小板聚集；以SDS－PAGE分离蛋白质，进行放射自显影；以TritonX－100抽提法沉淀骨架蛋白。结果：（1）1μmol／LStaurosporine完全抑制0．5U／ml凝血酶或10μmol／LPMA诱导的血小板聚集，大部分抑制20μmol／LA23187诱导的血小板聚集。（2）1μmol／LStaurosporine几乎完全抑制0．5U／ml凝血酶、或10μmol／LPMA、或20μmol／LA23187诱导的血小板40kD和20kD蛋白磷酸化。（3）1μmol／LStaurosporine完全抑制0．5U／ml凝血酶或10μmol／LPMA诱导的血小板肌动蛋白聚合。结论：Staurosporine抑制蛋白激酶C的活性．从而抑制血小板的聚集和肌动蛋白聚合；蛋白激酶C在血小板聚集和肌动蛋白聚合中起着重要调控作用。
Objective: To further investigate the role of protein kinase C inhibitor Staurosporine in platelet aggregation, protein phosphorylation and actin polymerization. Methods: Platelets were labeled with 32P-Na2HPO4; platelet aggregation was measured by platelet aggregation; proteins were separated by SDS-PAGE and autoradiographed; and cytoskeletal proteins were precipitated by Triton X-100 extraction. Results: (1) 1μmol / LStaurosporine completely inhibited platelet aggregation induced by 0.5U / ml thrombin or 10μmol / LPMA, most of which inhibited platelet aggregation induced by 20μmol / LA23187. (2) 1μmol / LStaurosporine almost completely inhibited 0.5U / ml thrombin, or 10μmol / LPMA, or 20μmol / LA23187 induced platelet 40kD and 20kD protein phosphorylation. (3) 1μmol / LStaurosporine completely inhibited 0.5U / ml thrombin or 10μmol / LPMA induced platelet actin polymerization. Conclusion: Staurosporine inhibits protein kinase C activity. Thereby inhibiting platelet aggregation and actin polymerization; protein kinase C plays an important regulatory role in platelet aggregation and actin polymerization.