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利用生物统计软件对兰州生研所１２年间生产的１４７批百日咳菌苗原液的免疫力进行质反应平行线测定分析（平行线法），并与《中国生物制品规程》９５版中ＷｉｌｓｏｎＷｏｒｃｅｓｔｅｒ法（Ｗ－Ｗ）比较，结果表明，质反应平行线测定法的各剂量反应百分率落在经验区间内；ＥＤ５０可信限区间为０．０１６～０．０１４；相对效价９５％可信限区间为０７７５～０．６５５。按合格、不合格、复试三个水平对１４７批百日咳菌苗的免疫力进行了分析与评价。并以中国百日咳菌苗免疫力试验用参考品为对照，对待检菌苗的稳定性及实验一致性进行了质量检测，计得总体回归系数分别为１．５８７９（ｘ２＝６０．９０）和１．３８４９（ｘ２＝１６３．２４）；总体ＥＤ５０分别为０．０１４和０．０１５；待检菌苗总体相对效价０７１４，两法的ＥＤ５０值间及相对效价值间存在很好的相关性（ｒ＝０．８３７６及ｒ＝０．９９４０）。
The bioassay software was used to measure the parallel reaction of the immunity of 147 B. pertussis vaccine strains produced by Lanzhou Institute of Health in 12 years (parallel line method) and compared with the WilsonWorcester method W-W). The results showed that the percentages of each dose response in the parallel reaction of mass reaction fell within the empirical range; the ED50 confidence interval was 0.016 ~ 0.014; the relative potency 95% confidence interval was 0 775 ~ 0.655. The immunity of 147 B. pertussis strains was analyzed and evaluated according to the three levels of pass, fail and retest. In order to compare the stability and experimental consistency of the tested vaccine against the pertussis vaccine in China, the overall regression coefficients were 1.5879 (x2 = 60.90) and 1 .3849 (x2 = 163.24). The overall ED50 were 0.014 and 0.015, respectively. The overall relative titer of the vaccine to be tested was 0.73. There was a good correlation between the ED50 values and relative efficacy values (R = 0.8376 and r = 0.9940).