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A simple protocol was established for DNA extraction using etiolated rice seedlings, whereby rice DNA was directly extracted in 0.5 mol/L NaOH solution in a single eppendorf tube. Results of comparative PCR analyses and electrophoresis showed that the DNA extracted using this method was as good and useful as that using standard CTAB method.
A simple protocol was established for DNA extraction using etiolated rice seedlings, so rice DNA was directly extracted in 0.5 mol / L NaOH solution in a single eppendorf tube. Results of comparative PCR analyzes and electrophoresis showed that the DNA extracted using this method was as good and useful as that using standard CTAB method.