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目的:观察补肾活血汤对去势后大鼠腰椎间盘髓核组织结构及病理形态变化以及对椎间盘p38MAPK蛋白表达的影响。方法:选取清洁级12月龄健康雌性SD大鼠60只,随机分为空白对照组、假模型组、模型组、中药组以及阳性药物组,每组12只大鼠。采用绝经后骨质疏松症动物造模方法,空白对照组、假模型组与模型组生理盐水灌胃,中药组予补肾活血汤灌胃。持续给药12周。分别于8周、12周后每组随机抽取6只大鼠处死取材,运用Western Blotting方法检测椎间盘中蛋白表达差异。结果:模型组椎间盘中p38MAPK蛋白含量高于空白对照组、假模型组(P<0.05);中药组p38MAPK蛋白含量低于模型组(P<0.05)。结论:补肾活血汤可抑制去势后大鼠腰椎间盘组织形态学的退变及p38MAPK蛋白表达,对椎间盘退变具有一定的延缓作用。
Objective: To observe the effect of Bushen Huoxue Decoction on nucleus pulposus nucleus pulposus structure change and pathomorphology in ovariectomized rats and its effect on expression of p38MAPK protein in intervertebral disc. Methods: Sixty healthy 12-month-old female Sprague-Dawley rats were randomly divided into blank control group, sham model group, model group, TCM group and positive drug group, with 12 rats in each group. Using animal modeling method of postmenopausal osteoporosis, the blank control group, the model group and the model group were given normal saline, while the TCM group was given Bushenhuoxue Decoction. Administered for 12 weeks. Six rats were randomly selected from each group at 8 and 12 weeks after sacrifice. Western Blotting was used to detect the protein expression in the intervertebral disc. Results: The content of p38MAPK protein in intervertebral disc of model group was higher than that of blank control group and sham model group (P <0.05). The content of p38MAPK protein in traditional Chinese medicine group was lower than that of model group (P <0.05). Conclusion: Bushen Huoxue Decoction can inhibit the degeneration of lumbar disc tissue and the expression of p38MAPK protein in ovariectomized rats, which may delay the degeneration of intervertebral disc.