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目的:建立蒲地灌肠液中总黄酮含量测定的紫外分光光度法。方法:采用硝酸铝显色法,以芦丁为总黄酮代表物,以蒲地灌肠液本体为空白对照,测定波长为510 nm,测定蒲地灌肠液中总黄酮的含量,并进行准确度、精密度、专属性、线性与范围、稳定性等方法学考察。结果:黄酮类化合物在12.72~76.32 mg/L的范围内,浓度与吸光度呈现良好的线性关系,线性回归方程为A=0.01224C-0.00451(r=0.99975),加样回收率平均值为100.95%(n=9,RSD%=1.37%),4批次蒲地灌肠液中总黄酮平均含量为0.234%、0.237%、0.243%、0.235%、0.233%、0.197%。结论:本方法简便、快速、准确、灵敏,可作为蒲地灌肠液中总黄酮的含量测定的方法。
Objective: To establish a UV spectrophotometric method for the determination of total flavonoids in Pudi enema. Methods: Using aluminum nitrate colorimetric method, taking rutin as the representative of total flavonoids, taking Puti enema body as blank control, the wavelength was 510 nm, the content of total flavonoids in Pudi enema liquid was determined, and the accuracy and precision , Specificity, linearity and scope, stability and other methodological study. Results: The linear range was 12.72 ~ 76.32 mg / L. The linear regression equation was A = 0.01224C-0.00451 (r = 0.99975). The average recoveries of flavonoids were 100.95% (n = 9, RSD% = 1.37%). The average content of total flavonoids in four batches of Puti enema was 0.234%, 0.237%, 0.243%, 0.235%, 0.233% and 0.197%. Conclusion: The method is simple, rapid, accurate and sensitive and can be used as a method for determination of total flavonoids in Pudi enema.