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本文建立了简便准确的比色法用以测定L-天冬氨酸-β-脱羧酶的活力。即L-丙氨酸经氧化生成的乙醛能与ρ-羟基联苯形成稳定的紫色化合物,在576.8nm处有最大吸收,以此定量生成的微量L-丙氨酸.
This article established a simple and accurate colorimetric method for the determination of L-aspartate -β-decarboxylase activity. Acetaldehyde formed by oxidation of L-alanine can form a stable purple compound with ρ-hydroxybiphenyl and has a maximum absorption at 576.8 nm, thereby quantifying trace amounts of generated L-alanine.