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苹果酸脱氢酶普遍存在于各种生物中,它负责催化草酰乙酸和苹果酸之间的相互转换。根据其辅酶的特异性和在细胞内的分布和生理功能的不同,苹果酸脱氢酶在高等植物中可以区分出不同的类型。依赖于NAD的细胞质型苹果酸脱氢酶(cyMDH)是其中研究较少的一类。报道了从小麦(TritiumaestivumL.)中克隆的一种cyMDH的部分cDNA序列,并命名为W-MDH1。进化树分析表明来自不同生物的MDH可能是通过基因复制形成的。W-MDH1在小麦的根、茎、叶中呈组成型表达。
Malate dehydrogenase is ubiquitous in a variety of organisms and is responsible for catalyzing the conversion between oxaloacetate and malate. Depending on the specificity of its coenzyme and its distribution within the cell and its physiological function, malate dehydrogenase can distinguish different types in higher plants. NAD-dependent cytoplasmic malate dehydrogenase (cyMDH) is one of the less studied. A partial cDNA sequence for a cyMDH cloned from wheat (Tritiumaestivum L.) was reported and named W-MDH1. Phylogenetic tree analysis showed that MDH from different organisms may be formed by gene duplication. W-MDH1 is constitutively expressed in the roots, stems and leaves of wheat.