论文部分内容阅读
AIM: To study the protective effect of non-mitogenic human acidic fibroblast growth factor (FGF) on cardiac oxidative injury in vivo. METHODS: Ventricular cardiomyocytes were isolated from 1-to 3-d-old neonatal SD mice and cultured in Dulbecco’s minimum essential medium supplemented with 15% fetal bovine serum under an atmosphere of 50 mL/L CO_2-95% air at 37°C, as well as assessed by immunocyto-chemical assay. We constructed the cardiomyocyte injury model by exposure to a certain concentration of H_2O_2. Cellular viability, superoxide dismutase (SOD) activity, leakage of maleic dialdehyde and anti-apoptosis effect were included to evaluate the cardiac protective effect of non-mitogenic human acidic FGF. RESULTS: Over 50% of the cardiomyocytes beat spontaneously on the 2~(nd) d of culture and synchronously beat after being cultured for 3 d. Forty-eight hours after plating was completed, the purity of such cultures was 95% mybcytes, assessed by an immunocytochemical assay. Cellular viability dramatically decreased with the increasing of the concentration of H_2O_2. Non-mitogenic human acidic FGF showed significant resistance to the toxic effect of H_2O_2, significantly increased the cellular viability as well as the activity of SOD, and dramatically decreased the leakage of maleic dialdehyde as well as the cellular apoptosis rate. CONCLUSION: Hydrogen peroxide shows strong cytotoxicity to the cultured cardiac myocytes, and non-mitogenic human acidic FGF shows strong cardio-protective effect when exposed to a certain concentration of H_2O_2.
METHODS: Ventricular cardiomyocytes were isolated from 1-to 3-d-old neonatal SD mice and cultured in Dulbecco’s minimum essential medium supplemented with 15% fetal bovine serum under an atmosphere of 50 mL / L CO 2-95% air at 37 ° C, as well as assessed by immunocyto-chemical assay. We constructed the cardiomyocyte injury model by exposure to a certain concentration of H 2 O 2 Over 50% of the cardiomyocytes beat spontaneously on the 2 ~ (nd) d of culture and synchronously beat after being cultured for 3 d. Forty-eight hours after plating was completed, the purity of such cultures was 95% mybcytes, assessed by an immunocytochemical assay. Cellular via bility dramatically decreased with the increasing of the concentration of H 2 O 2. Non-mitogenic human acidic FGF showed significant resistance to the toxic effect of H 2 O 2, significantly increased the cellular viability as well as the activity of SOD, and dramatically decreased the leakage of maleic dialdehyde as well as the cellular apoptosis rate. CONCLUSION: Hydrogen peroxide shows strong cytotoxicity to the cultured cardiac myocytes, and non-mitogenic human acidic FGF shows strong cardio-protective effect when exposed to a certain concentration of H 2 O 2.