Content Determination of β-element in Different Parts of Eupatorium adenophorum Spreng

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[Objective]To determine the contents of β-elemene in different parts of Eupatorium adenophorum Spreng. [Method]Contents of β-el-emene in different parts of E. adenophorum grown in Panxi Area of China were measured by HPLC. The chromatographic conditions were as follows: Eclipse XDB-C18( 4.6 mm ×150 mm,5 μm) ,1.0 ml /min flow rate,210 nm detective wavelength,ethanol-acetonitrile-water as ( V/V/V,70∶ 10∶ 20) mobile phase,20 μl injection volume,and 30 ℃ column temperature. [Result]β-elemene existed in different parts of E. adenophorum plant. And the content was the maximum in leaves. In the natural drying leaf sample,β-elemene content was 0. 083%,which was more than 3 times higher than the content in Curcuma wenyujin Y. H. Chen. [Conclusion]Extracting β-elemene from E. adenophorum could greatly reduce the material cost of β-elemene,and could develop new methods for the control of malignant weeds in China. [Objective] To determine the contents of β-elemene in different parts of Eupatorium adenophorum Spreng. [Method] Contents of β-el-emene in different parts of E. adenophorum grown in Panxi Area of ​​China were measured by HPLC. The chromatographic conditions (4.6 mm × 150 mm, 5 μm), 1.0 ml / min flow rate, 210 nm detective wavelength, ethanol-acetonitrile-water as (V / V / V, 70:10:20) mobile phase, 20 μl injection volume, and 30 ° C column temperature. [Result] β-elemene existed in different parts of E. adenophorum plant. And the content was the maximum in leaves. In the natural drying leaf sample, β-elemene content was 0. 083%, which was more than 3 times higher than the content in Curcuma wenyujin YH Chen. [Conclusion] Extracting β-elemene from E. adenophorum could greatly reduce the material cost of β-elemene, and could develop new methods for the control of malignant weeds in China.
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