论文部分内容阅读
目的:探讨子宫颈上皮内瘤变及子宫颈鳞癌中抑制基因蛋白(p16)、增殖细胞核蛋白(Ki-67)表达及其意义。方法:应用免疫组化S-P法,检测子宫颈鳞癌14例、子宫颈上皮内瘤变77例、正常子宫颈组织23例中p16、Ki-67表达水平,并进行分析。结果:(1)p16在子宫颈上皮内瘤变组和子宫颈鳞癌组中阳性表达率明显高于子宫颈正常组(P<0.01)。Ki-67在子宫颈上皮内瘤变和子宫颈鳞癌组中阳性表达率明显高于子宫颈正常组(P<0.01)。(2)从正常子宫颈组织、子宫颈上皮内瘤变到子宫颈鳞癌,Ki-67的表达强度逐步增强,两者呈正相关(P<0.01)。(3)在子宫颈上皮内瘤变和子宫颈鳞癌中,p16、Ki-67间无相关性(P>0.05)。结论:p16、Ki-67参与了子宫颈上皮内瘤变、子宫颈鳞癌的发生。p16、Ki-67联合检测作为诊断子宫颈癌前病变及子宫颈鳞癌的指标,可提高子宫颈鳞癌的早期诊断率。
Objective: To investigate the expression of suppressor gene protein (p16) and proliferating cell nuclear protein (Ki-67) in cervical intraepithelial neoplasia and cervical squamous cell carcinoma and its significance. Methods: The expression of p16 and Ki-67 in 14 cases of cervical squamous cell carcinoma, 77 cases of cervical intraepithelial neoplasia and 23 cases of normal cervical tissue were detected by immunohistochemical S-P method and analyzed. Results: (1) The positive rate of p16 in cervical intraepithelial neoplasia group and cervical squamous cell carcinoma group was significantly higher than that in normal cervix group (P <0.01). The positive rate of Ki-67 in cervical intraepithelial neoplasia and cervical squamous cell carcinoma was significantly higher than that in normal cervix (P <0.01). (2) The expression intensity of Ki-67 gradually increased from normal cervical tissue, cervical intraepithelial neoplasia to cervical squamous cell carcinoma, which had a positive correlation (P <0.01). (3) There was no correlation between p16 and Ki-67 in cervical intraepithelial neoplasia and cervical squamous cell carcinoma (P> 0.05). Conclusion: p16 and Ki-67 are involved in the pathogenesis of cervical intraepithelial neoplasia and cervical squamous cell carcinoma. p16, Ki-67 joint detection as a diagnostic indicator of cervical precancerous lesions and cervical squamous cell carcinoma can improve the early diagnosis of cervical squamous cell carcinoma.