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本实验通过向密闭舱内灌注混合气体的方法，分别复制慢性缺氧、慢性缺氧伴二氧化碳潴留及慢性呼吸性酸中毒大鼠模型。以地高辛标记的脑阴离子转运蛋白（ＡＥ３）ｃＤＮＡ探针，应用核酸原位杂交及点杂交技术，对ＡＥ３ｍＲＮＡ的分布及变化进行检测，结果显示：（１）ＡＥ３ｍＲＮＡ广泛分布于脑组织，其中以呼吸调整中枢所在部位脑桥深部灰质最为富集；（２）慢性缺氧组ＡＥ３ｍＲ－ＮＡ的相对含量显著地低于对照组（Ｐ＜０．０５），慢性缺氧伴二氧化碳潴留及慢性呼吸性酸中毒组均非常显著地低于对照组（Ｐ＜０．０１）；（３）慢性缺氧伴二氧化碳潴留及慢性呼吸性酸中毒组均非常显著地低于慢性缺氧组（Ｐ＜０．０１），但二组之间无显著性差异。提示ＡＥ３在呼吸衰竭的发生及其导致的肺性脑病中可能具有一定作用。
In this experiment, the model of chronic hypoxia, chronic hypoxia with carbon dioxide retention and chronic respiratory acidosis were duplicated by perfusion of mixed gas into airtight compartments. The cDNA of AE3 mRNA labeled with digoxigenin was detected by in situ hybridization and dot blot hybridization. The results showed that: (1) AE3 mRNA was widely distributed in brain tissue, of which (2) The relative content of AE3mR-NA in chronic hypoxia group was significantly lower than that in control group (P <0.05), chronic hypoxia with carbon dioxide retention and chronic respiratory Acidosis group were significantly lower than the control group (P <0.01); (3) chronic hypoxia with carbon dioxide retention and chronic respiratory acidosis group were significantly lower than the chronic hypoxia group (P <0. 01), but no significant difference between the two groups. These results suggest that AE3 may play a role in the pathogenesis of respiratory failure and pulmonary encephalopathy.