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将ＢＡＬＢ／ｃ 小鼠的Ｈ２２ 腹水型肝癌和６１５ 系小鼠的Ｌ６１５ 白血病细胞经榄香烯或／和热休克等不同处理制成瘤苗， 进行体内主动免疫实验和致敏脾细胞的体外细胞毒活性实验。结果表明： 热休克和榄香烯复合瘤苗比单一因素处理的瘤苗免疫效果好， 经Ｈ２２和Ｌ６１５ 复合瘤苗免疫、攻击后两个月动物存活率分别达６０－０％ 、８５－０％ （ Ｐ＜０－０１）， 并且能够明显提高死亡小鼠的平均存活天数。瘤苗免疫动物脾细胞的细胞毒活性分别达５８－８％ 、６９－７％ （ Ｐ ＜ ０－０１） 。在制备复合瘤苗时先加入榄香烯后再热休克的免疫效果较好， 这在Ｌ６１５ 瘤苗更为明显， 从而为今后瘤苗免疫在临床上的应用提供了进一步的实验依据。
H22 ascites hepatocellular carcinoma of BALB / c mice and L615 leukemia cells of 615 mice were treated with different treatments of elemene and / or heat shock to make tumor vaccine, in vivo active immune experiments and in vitro cell-sensitization of splenocytes Toxicity test. The results showed that the heat shock and elemene composite tumor vaccine had a better immunization effect than the single factor vaccine. After being vaccinated with the H22 and L615 tumor vaccine, the survival rates of the two months after the challenge were 60-0% and 85-0 % (P <0-01), and can significantly increase the average survival days of the dead mice. The cytotoxic activity of splenocytes of immunized mice was 58-8% and 69-7%, respectively (P <0-01). In the preparation of composite tumor vaccine first elemene after heat shock better immune effect, which is more obvious L615 tumor vaccine, so as to future vaccine immunization in clinical application provides further experimental basis.