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目的研究大肠肿瘤组织中Bmi-1、P16蛋白表达情况及临床意义。方法采用免疫组织化学方法分别检测Bmi-1、p16蛋白在60例大肠癌、30例大肠腺瘤及20例正常大肠粘膜组织中的表达情况及其与大肠癌临床病理特征及患者生存率的关系。结果Bmi-1及p16蛋白在大肠癌中表达明显高于腺瘤组及正常组(P<0.05);采用卡方检验显示,Bmi-1蛋白高表达与有无远处转移密切相关(P<0.01),与TNM分期有关(P<0.05),而p16蛋白表达与各临床病理特征无关(P>0.05);Kaplan-Meier生存分析显示Bmi-1蛋白高表达患者生存率明显低于低表达患者(P<0.05),而p16蛋白表达阳性患者生存率与p16蛋白表达阴性患者生存率差异无统计学意义(P>0.05)。结论Bmi-1蛋白表达与大肠癌的发生、发展及预后关系密切,可作为评估大肠癌预后的参考指标;p16蛋白表达可能与大肠癌发生有一定关系,但与肿瘤转移及预后无关。
Objective To study the expression of Bmi-1 and P16 in colorectal tumor and its clinical significance. Methods The expressions of Bmi-1 and p16 proteins in 60 colorectal carcinomas, 30 colorectal adenomas and 20 normal colorectal mucosa tissues were detected by immunohistochemistry and their correlation with the clinicopathological characteristics and survival rate of colorectal cancer . Results The expression of Bmi-1 and p16 protein in colorectal carcinoma was significantly higher than that in adenoma group and normal group (P <0.05). The chi-square test showed that the high expression of Bmi-1 protein was closely related to the distant metastasis (P < 0.01), but not with the clinicopathological features (P> 0.05). The Kaplan-Meier survival analysis showed that the survival rate of patients with high expression of Bmi-1 protein was significantly lower than that of patients with low expression (P <0.05). However, there was no significant difference in the survival rate between patients with positive p16 protein expression and those with negative p16 protein expression (P> 0.05). Conclusion The expression of Bmi-1 protein is closely related to the occurrence, development and prognosis of colorectal cancer, which may be used as a reference index to evaluate the prognosis of colorectal cancer. The expression of p16 protein may be related to the occurrence of colorectal cancer, but not to the metastasis and prognosis.