论文部分内容阅读
目的探讨南宁市肠出血性O157大肠埃希氏菌人感染、动物和媒介昆虫带菌状况。方法2008年所采集的腹泻病人粪便、动物粪便和苍蝇等标本,经处理后接种mEC肉汤增菌,先用免疫胶体金标法做初筛,阳性标本用免疫磁珠浓缩目标菌,划线接种CHROMagarO157显色琼脂平板,纯化后进行血清学诊断,然后集中进行系统生化鉴定和毒力检测等。结果共检测1083份标本,检出10株O157大肠埃希菌,总阳性率为0.92%,其中猪粪便为9.38%,牛粪为4.00%,苍蝇为4.35%,鸡粪为0.49%,腹泻病人粪便和鸭粪均未能出O157大肠埃希菌,部分标本中检出与O157大肠埃希菌有相关抗原的致病性大肠埃希菌(EPEC)和未能定型的大肠埃希氏菌;对所检出10株O157菌做SLT1、SLT2、eaeA、hly44种毒力基因的检测,发现3株菌(同一猪场的1份猪粪便和2份苍蝇中检出)eaeA+hly44阳性,未见含有SLT1SLT2基因,其他菌株SLT1SLT2eaeAhly44均为阴性。结论南宁市养殖场部分动物体携带有一定程度致病力的0157大肠埃希菌,并已造成了周围环境污染,威胁人们的生命健康,提示加强宿主动物0157大肠埃希菌监测和研究是防止未来大流行的关键。
Objective To investigate the human infection of enterohemorrhagic O157 Escherichia coli in Nanning City and the status of infection by animals and vector insects. Methods The specimens of excrement, animal faeces and flies collected from diarrhea patients in 2008 were treated and inoculated with mEC broth. The samples were screened by immunogold gold standard method. The positive samples were concentrated with immunomagnetic beads and scribed Inoculation CHROMagarO157 color agar plate, purified serological diagnosis, and then focused on systematic biochemical identification and virulence testing. Results A total of 1083 specimens were detected and 10 strains of O157 Escherichia coli were detected with a positive rate of 0.92%, of which pig manure was 9.38%, cattle manure was 4.00%, flies were 4.35%, chicken manure was 0.49%, diarrhea patients Stool and duck excrement were unable to produce O157 Escherichia coli, some of the specimens were detected with O157 Escherichia coli-associated antigen pathogenic Escherichia coli (EPEC) and failed to type Escherichia coli; The detection of 10 virulence genes of SLT1, SLT2, eaeA and hly among 10 strains of O157 detected showed that 3 strains (detected in 1 pig feces and 2 flies in the same farm) were positive for eaeA + hly44 See also contains SLT1SLT2 gene, other strains SLT1SLT2eaeAhly44 were negative. Conclusion Some of the animals in Nanning farm carry a certain degree of virulence of 0157 Escherichia coli and have caused the pollution of the surrounding environment and threaten the life and health of human beings, suggesting that monitoring and research on strengthening the host animal 0157 Escherichia coli is to prevent The key to a future pandemic.