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以前阿霉素(DOX)与单克隆抗体(mAb)的免疫偶联物对杀伤肿瘤细胞的研究多局限于异体移植的皮下肿瘤,本文研究了DOX免疫偶联物对自发转移性人黑色素瘤的治疗效果。 将DOX与抗人表皮细胞生长因子受体(EGFR)单抗mAb425以成二醛法连接成免疫偶联物425-DOX,并检测了其体内、外抑制人黑色素瘤细胞系M24met细胞的生长效应;以M24met细胞100μl(2×10~6个)皮下
Previous studies of the immunoconjugates of doxorubicin (DOX) and monoclonal antibodies (mAbs) to kill tumor cells were mostly limited to allograft subcutaneous tumors. This article investigated the effect of DOX immunoconjugates on spontaneous metastatic human melanoma. treatment effect. The DOX and anti-human epidermal growth factor receptor (EGFR) mAb mAb425 were ligated into the immunoconjugate 425-DOX by dialdehyde method, and the growth effect of the human melanoma cell line M24met cells was detected in vitro and in vivo. 100μl (2×10~6) subcutaneously with M24met cells