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一、引言Bouma 和 Dowling(1982)发表了测定地三叶草(Trifolium subterraneum)近期磷素状况的一种迅速而简单的方法。它无需实验室设备,也不需要专门的技能。因此,适合在农场应用。这种方法主要是用几滴10NH_2SO_4,萃取新鲜叶片组织。经过进一步的研究,Irving 和 Bouma(1984)指出,这种方法提供了取样时绿色叶片组织中无机磷(Pi)的可靠的估计值,而不因有机磷化合物水解而产生干扰。
I. INTRODUCTION Bouma and Dowling (1982) published a quick and easy method to determine the recent status of phosphorus in the clover (Trifolium subterraneum). It does not require lab equipment and does not require specialized skills. Therefore, suitable for farm applications. This method is mainly to use a few drops of 10NH_2SO_4 extract fresh leaf tissue. After further study, Irving and Bouma (1984) pointed out that this method provides a reliable estimate of inorganic Pi in green leaf tissue at sampling without disturbing the hydrolysis of organophosphorus compounds.