论文部分内容阅读
目的:调查H血型抗原在人肿瘤细胞系BEL-7404、SPC-A-1和SGC-7901上的表达.方法:免疫组织化学和流式细胞术分析细胞上H抗原在体内外的表达.RT-PCR,Southern印迹和限制性酶切分析确定FUT2基因在细胞中的表达.结果:SGC-7901,BEL-7404和SPC-A-1细胞表面H抗原表达的平均荧光强度分别为162±43,81±25和28±17.并且用RT-PCR从这些细胞中扩增出(?)约1.0 kb DNA条带,此条带能被FUT2特异核酸探针检出.结论:人肿瘤细胞系BEL-7404,SPC-A-1和SGC-7901于体内外在细胞表面均表达H抗原,但表达水平在细胞之间变化显著.FUT2基因的表达在细胞膜上产生这些抗原.
Objective: To investigate the expression of H antigen on human tumor cell lines BEL-7404, SPC-A-1 and SGC-7901.Methods: Immunohistochemistry and flow cytometry were used to analyze the expression of H antigen in vitro and in vivo.RT The expression of FUT2 gene in cells was determined by PCR, Southern blot and restriction enzyme digestion.Results: The average fluorescence intensity of H antigen expression on SGC-7901, BEL-7404 and SPC-A-1 cells was 162 ± 43, 81 ± 25 and 28 ± 17, and about 1.0 kb DNA bands were amplified from these cells by RT-PCR, and the bands could be detected by FUT2 specific nucleic acid probes.Conclusion: The human tumor cell line BEL -7404, SPC-A-1 and SGC-7901 both expressed H antigen on the cell surface both in vitro and in vivo, but the expression levels varied significantly among cells.The expression of the FUT2 gene produced these antigens on the cell membrane.