论文部分内容阅读
目的:优选叠鞘石斛联苄类化合物的大孔树脂纯化工艺条件并考察其体外抗氧化活性。方法:以静态饱和吸附量和洗脱率为指标,通过静态吸附-洗脱试验筛选大孔树脂型号。采用单因素试验考察上样液质量浓度、上样量、乙醇体积分数、洗脱剂用量、上样流速及洗脱流速对纯化工艺的影响,通过体外抗氧化试验考察联苄类化合物的体外抗氧化能力。结果:HPD-300型树脂纯化联苄类化合物的效果最好,其最佳纯化工艺条件为上样液质量浓度9.04 g·L-1,上样量1.25 BV,上样流速0.5 mL·min-1,加水和70%乙醇各5 BV以1.0 mL·min-1的流速洗脱。纯化后联苄类化合物纯度达67.07%,较纯化前提高了4.25倍。联苄类化合物的纯化物和粗提物对超氧阴离子自由基的抗氧化活性以半数抑制浓度(IC50)表示分别为0.408,0.985 g·L-1,对羟自由基的IC50依次为7.856,7.827 g·L-1。结论:HPD-300型大孔吸附树脂对叠鞘石斛联苄类化合物的纯化效果较好,纯化物的体外抗氧化能力有所提高。
OBJECTIVE: To optimize the macroporous resin purification process condition of Dendrobium candidum and its anti-oxidative activity in vitro. Methods: Based on the static adsorption capacity and elution rate, macroporous resin was screened by static adsorption - elution test. The single factor test was used to investigate the effects of the concentration of the sample solution, the sample volume, the ethanol volume fraction, the eluent dosage, the sample flow rate and the elution flow rate on the purification process. The in vitro anti-oxidation test Oxidation ability. Results: HPD-300 resin was the best for the purification of bibenzyl compounds. The optimal purification conditions were as follows: the mass concentration of sample solution 9.04 g · L-1, the sample volume 1.25 BV, the sample flow rate 0.5 mL · min- 1, 5 BV with water and 70% ethanol at a flow rate of 1.0 mL · min -1. Purified bibenzyl compounds purity of 67.07%, 4.25 times higher than before purification. Antioxidant activity of the bibenzyl compounds and crude extracts on superoxide anion free radicals was expressed at IC50 of 0.408 and 0.985 g · L -1, IC50 of hydroxyl radical was 7.856, 7.827 g · L -1. CONCLUSION: The HPD-300 macroporous resin has good purification effect on the bibenzyls of D. scoparia. The purification ability of the purified product in vitro is improved.