论文部分内容阅读
水稻突变体ys94,是一个来源于化学诱变的叶色突变体。突变体在三叶期前出现明显的黄叶表型,叶绿素a、叶绿素b含量下降;透射电镜观察表明,突变体叶片叶绿体数量减少、类囊体片层退化。三叶期后叶色开始转绿。成熟期突变体ys94的分蘖数减少,但在结实率和株高等性状上与野生型相比并无明显差异。遗传分析表明,ys94的突变性状由一对隐性核基因控制。利用ys94×DJY组合的F_2定位群体,将突变基因定位在第8染色体短臂标记M8-3和M8-24之间,两标记间的物理距离为140kb,此区间包含11个候选基因。本研究结果为候选基因的克隆和功能分析奠定了基础。
The rice mutant ys94, a leaf color mutant derived from chemical mutagenesis, The mutant showed significant yellow leaf phenotype before the three-leaf stage, the content of chlorophyll a and chlorophyll b decreased. The number of chloroplast in the mutant decreased and the thylakoid lamella degenerated. After the leaf clover color began to turn green. There was no significant difference in the number of tillers in mature stage ys94 between the two cultivars but in seed setting rate and plant height traits. Genetic analysis showed that the mutation of ys94 was controlled by a pair of recessive nuclear genes. The F2 locating population of ys94 × DJY was used to map the mutant gene between M8-3 and M8-24 on the short arm of chromosome 8. The physical distance between the two markers was 140kb, and 11 candidate genes were located in this interval. The results of this study laid the foundation for the cloning and functional analysis of candidate genes.