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目的研究人乳头瘤状病毒(HPV)在新乡地区食管鳞癌、癌旁组织中的感染情况和人类白细胞抗原系统G(HLA-G)表达的关系。方法采用基因芯片技术检测114例食管鳞癌组织及癌旁食管正常组织中HPV亚型感染情况,免疫组化SP法检测HLA-G的表达。结果 114例食管鳞癌组织及癌旁食管正常组织中HPV感染阳性率分别为63.2%和6.0%,HLA-G的检出率分别为51.8%、0%,差异均有统计学意义(P<0.05);食管鳞癌存在HPV16、18和52三种亚型感染,其中HPV16感染率最高,HPV52感染率最低。结论新乡地区食管鳞癌组织以HPV16、18和52亚型感染为主;HPV感染和HLA-G蛋白表达与食管鳞癌分化程度正相关;HPV感染食管鳞癌组织与HLA-G蛋白表达存在一定的相关性,可能提示与食管鳞癌发生有较密切关系。
Objective To study the relationship between the infection of human papillomavirus (HPV) in esophageal squamous cell carcinoma and paracancerous tissue in Xinxiang area and the expression of human leukocyte antigen system G (HLA-G). Methods The genotypes of 114 cases of esophageal squamous cell carcinoma and adjacent normal esophageal squamous cell carcinoma were used to detect HPV subtypes. The expression of HLA-G was detected by immunohistochemical SP method. Results The positive rates of HPV infection in 114 esophageal squamous cell carcinoma tissues and adjacent normal esophageal tissues were 63.2% and 6.0%, respectively. The positive rates of HLA-G were 51.8% and 0%, respectively, with significant difference (P < 0.05). Esophageal squamous cell carcinoma (HPV16, HPV18) and subtype 52 (HPV16, HPV18) were the most common subtypes. The HPV16 infection rate was the highest and HPV52 infection rate was the lowest. Conclusions HPV16, 18 and 52 subtypes are the major pathogens of esophageal squamous cell carcinoma in Xinxiang area. HPV infection and HLA-G protein expression are positively correlated with the differentiation of esophageal squamous cell carcinoma. The expression of HLA-G protein in HPV-infected esophageal squamous cell carcinoma The correlation may be prompted with the occurrence of esophageal squamous cell carcinoma are more closely related.