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AIM: To observe the effect of compound Biejiaruangandecoction (CBJRGC) (composite prescription of Carapaxtrionycis for softening the liver) on proliferation,activation,excretion of collagen and cytokine of hepatic stellate cells(HSCs) and to find the mechanism of prevention andtreatment of hepatic fibrosis by CBJRGC.METHODS: Using MTI,immunohistochemistry and imageanalysis technology,the related indexes for proliferation,activation,excretion of collagen and cytokine of hepatic stellatecells were detected in 24 h,48 h,and 72 h after adminstrationof different dosages of CBJRGC.RESULTS: Statistical analysis showed that serum collectedfrom rat perfused with CBJRGC could restrain theproliferation of HSC in 48 h and 72 h especially in high andmedium dosage groups,markedly decrease the expressionof desmin,synapsin and platelet derived growth factor(PDGF) in HSC in 24 h,48 h and 72 h,as well as theexpression of α-SMA,collagen Ⅲ,TIMP and TGFβ1 in 48 hand 72 h,decrease the excretion of collagen Ⅰ in 72 h.CBJRGC serum had no significant effect on collagens Ⅰ,Ⅲand TIMP in 24 h.CONCLUSION: CBJRGC serum has a good curative effecton hepatic fibrosis.Its main mechanism may be related tothe following factors.The drug serum can restrain theproliferation and activation of HSC,decrease the number ofactivated HSC and the total number of HSC,the excretion ofcollagens Ⅰ,Ⅲ,enhance the degradation of collagen andrestore the balance of synthesis and degradation of collagen,inhibit the expression of transforming growth factor β1(TGFβ1) and platelet derived growth factor (PDGF) in HSC,block and delay the process of hepatic fibrosis.Synapsin isa new marker of activation of HSC,which provides atheoretical and testing basis for neural regulation in thedeveloping process of hepatic fibrosis.
AIM: To observe the effect of compound Biejiaruangandecoction (CBJRGC) (composite prescription of Carapaxtrionycis for softening the liver) on proliferation, activation, excretion of collagen and cytokine of hepatic stellate cells (HSCs) and to find the mechanism of prevention and treatment of hepatic fibrosis by CBJRGC.METHODS: Using MTI, immunohistochemistry and imageanalysis technology, the related indexes for proliferation, activation, excretion of collagen and cytokine of hepatic stellate cells were detected in 24 h, 48 h, and 72 h after adminstration of different dosages of CBJRGC.RESULTS: Statistical analysis showed that serum collected from rat perfused with CBJRGC could restrain the proliferation of HSC in 48 h and 72 h especially in high and medium dosage groups, markedly decrease the expression of desmin, synapsin and platelet derived growth factor (PDGF) in HSC in 24 h, 48 h and 72 h, as well as the expression of α-SMA, collagen Ⅲ, TIMP and TGFβ1 in 48 hand 72 h, decrease the excretion of colla gen I in 72 h. CBJRGC serum had no significant effect on collagens I, III and TIMP in 24 h. CONCLUSION: CBJRGC serum has a good curative effect on hepatic fibrosis. It’s main mechanism may be related to the following factors. The drug serum can restrain the proliferation and activation of HSC, decrease the number ofactivated HSC and the total number of HSCs, the excretion ofcollagens I, III, enhance the degradation of collagen andrestore the balance of synthesis and degradation of collagen, inhibit the expression of transforming growth factor β1 (TGFβ1) and platelet derived growth factor (PDGF) in HSC, block and delay the process of hepatic fibrosis. Synapsin isa new marker of activation of HSC, which provides atheticaltical and testing basis for neural regulation in the development process of hepatic fibrosis.