论文部分内容阅读
AIM:To examine whether glutamine prevents the injuryto the intestinal mucosa after intestinal ischemia-reperfusion(I/R)in rats.METHODS:Thirty male Sprague-Dawley rats were randomlydivided into 3 groups:a standard parenteral nutrition(PN)group(n=10);an I/R-PN group(n=10);an I/R-glutamineendohed PN(I/R-GIn)group(n=10).The superior mesentedcartery(SMA)was clamped.After 60 rain of ischemia,reperfusion was initiated and infusion was started.All ratsreceived isocaloric and isonitrogenous nutritional supportfor 48 h.Spleen,liver,mesenteric lymph nodes(MLN),andintestinal segments were removed for morphological andbiochemical analyses,and blood samples were collectedfor bacterial culture and measurement of endotoxin levels.The permeability of intestinnal mucosa was assayed bymeasurement of D-(-)-Iactate levels in plasma.RESULTS:In I/R-PN group,extensive epithelial atrophywas observed,mucosal thickness,villous height,crypt depthand villous surface area were decreased significantly comparedwith PN group,whereas these findings did not occur in theI/R-GIn group.The incidence of intestinal bacterialtranslocation to spleen,liver,MLN,and blood wassignificantly higher in I/R-PN group than that in other groups.Plasma endotoxin levels significantly increased in the I/R-PNgroup compared with the I/R-GIn group.Remarkably highervalues of D-(-)-Iactate were also detected in PN groupcompared with that in I/R-GIn group.CONCLUSION:Glutamine protects the morphology andfunction of intestinal mucosa from injury after I/R in rats.
AIM: To examine whether glutamine prevents the injury to the intestinal mucosa after intestinal ischemia-reperfusion (I / R) in rats. METHODS: Thirty male Sprague-Dawley rats were differentiated into 3 groups: a standard parenteral nutrition (PN) group 10); an I / R-PN group (n = 10); an I / R-glutamineendohed PN (I / R- GIn) group (n = 10); superior superior cartilage (SMA) was clamped. ischemia, reperfusion was initiated and infusion was started. All rats are naturally isocaloric and isonitrogenous nutritional support for 48 h. Spleen, liver, mesenteric lymph nodes (MLN), and intestinal segments were removed for morphological and biochemical analyzes, and blood samples were collected for bacterial culture and measurement of endotoxin levels. The permeability of intestinnal mucosa was assayed by measurement of D - (-) - Iactate levels in plasma .RESULTS: In I / R-PN group, extensive epithelial atrophywas observed, mucosal thickness, villous height, crypt depthand villous surface area were projectedblywithP N group, yet these findings did not occur in the I / R-GIn group. The incidence of intestinal bacterial translocation to spleen, liver, MLN, and blood wassignificantly higher in I / R-PN group than that in other groups. increased in the I / R-PNgroup compared with the I / R-GIn group. Remarkably highervalues of D - (-) - Iactate were also detected in PN groupcompared with that in I / R-GIn group.CONCLUSION: Glutamine protects the morphology andfunction of intestinal mucosa from injury after I / R in rats.