论文部分内容阅读
目的:制备酚-黄复方微乳并考察其体外透皮扩散特性。方法:以油酸乙酯为油相,聚山梨酯-80(tween-80)为乳化剂,聚乙二醇-400(PEG-400)为助乳化剂,水相逐步加入到含药混合溶液中,制备酚-黄复方微乳。测定微乳的外观形态、粒径分布及稳定性。采用Franze扩散池法,考察补骨脂酚、黄芩苷在溶液和微乳中经皮渗透特性。结果:制备的微乳澄清透明,呈近球形,平均粒径(17.30±0.17)nm,稳定性较好。黄芩苷在溶液和微乳中24 h累积透过量(Q24 h)分别为(208.80±5.26),(232.38±15.07)μg·cm-2,经皮渗透特性无显著性差异。补骨脂酚24 h累积透过量由溶液中(324.81±56.63)μg·cm-2增加至微乳中的(721.30±108.88)μg·cm-2,增加了2.22倍,稳态渗透速率和表观渗透系数为溶液组的5.0倍,均有显著提高。结论:微乳经皮给药系统具有很好的促透作用,为酚-黄复方皮肤给药新制剂的设计提供实验依据。
OBJECTIVE: To prepare phenol-Huang compound microemulsion and study its in vitro transdermal diffusion characteristics. Methods: Ethyl oleate as the oil phase, polysorbate-80 (tween-80) as emulsifier and polyethylene glycol-400 as the co-emulsifier. The aqueous phase was gradually added to the drug- In the preparation of phenol - yellow composite microemulsion. Determination of the appearance of microemulsion size, particle size distribution and stability. Franze diffusion cell method was used to investigate the transdermal permeation characteristics of bakuchiol and baicalin in solution and microemulsion. Results: The prepared microemulsion was clear and transparent with nearly spherical shape and average particle size (17.30 ± 0.17) nm with good stability. The cumulative permeation of baicalin in solution and microemulsion for 24 h was (208.80 ± 5.26) and (232.38 ± 15.07) μg · cm-2, respectively, with no significant difference in percutaneous permeability. The cumulative permeate flux of bakuchiol increased from (324.81 ± 56.63) μg · cm-2 to (721.30 ± 108.88) μg · cm-2 in the microemulsion by 2.22 times, and steady state permeation rate Viewpoint osmotic coefficient of 5.0 times the solution group, both significantly increased. Conclusion: The system of transdermal delivery of microemulsion has a good penetration enhancement effect and provides experimental basis for the design of a new preparation of phenol - Huang compound cutaneous administration.