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目的 :调查上海市2013年新发现的经异性性传播的人类免疫缺陷病毒-1型(human immunodeficiency virus-1,HIV-1)感染者病毒亚型分布情况,为探索艾滋病防治策略提供思路。方法 :对289份经异性性传播HIV-1感染者的血浆标本,应用反转录-聚合酶链反应(reverse transcription polymerase chain reaction,RT-PCR)扩增后进行DNA测序,使用HIV-Blast软件判定其亚型,并结合流行病学资料进行分析。结果 :共获得289份pol区基因片段,亚型分布显示,CRF01_AE构成比最大,为54.3%;其次为CRF07_BC,为28.7%;B亚型为7.3%;CRF55_01B为4.2%;CRF08_BC为3.5%。其中,单纯经异性性接触传播的男性感染者有168例,占男性感染者总数的65.1%(168/258),亚型分布以CRF01_AE为主,占56.0%(94/168);异性性接触合并有男男同性接触史的男性感染者有83例,占32.2%(83/258),亚型分布也以CRF01_AE为主,占61.4(51/83),略高于单纯异性接触者;异性性接触合并注射毒品途径感染者7例,占2.7%(7/258),其亚型分布以CRF07_BC为主,有6例(6/7)。结论:上海市2013年经异性性传播HIV-1感染者的亚型分布以CRF01_AE和CRF07_BC为主,但在异性性传播伴有注射吸毒史的感染者中则以CRF07_BC亚型为主。
Objective: To investigate the distribution of the virus subtypes of newly discovered human immunodeficiency virus-1 (HIV-1) in Shanghai in 2013 and to provide ideas for exploring strategies for AIDS prevention and control. Methods: A total of 289 plasma samples from heterosexual HIV-1 infected individuals were amplified by reverse transcription polymerase chain reaction (RT-PCR) and sequenced. HIV-Blast software To determine the subtype, combined with epidemiological data for analysis. RESULTS: A total of 289 pol region gene fragments were obtained. The distribution of CRF01_AE was the largest with a ratio of 54.3%, followed by CRF07_BC with 28.7%, subtype B with 7.3%, CRF55_01B with 4.2% and CRF08_BC with 3.5%. Among them, 168 were infected by heterosexual contact alone, accounting for 65.1% (168/258) of the total number of men infected with HIV. The majority of subtypes were CRF01_AE, accounting for 56.0% (94/168). Heterosexual contact There were 83 cases of male infections with history of same sex with men, accounting for 32.2% (83/258). The distribution of subtypes was also mainly CRF01_AE, accounting for 61.4% (51/83), slightly higher than that of single heterosexual contacts There were 7 cases (2.7%) of patients infected by sexual contact and injecting drugs. The subtypes were mainly CRF07_BC, with 6 cases (6/7). Conclusion: The distribution of subtypes of HIV-1 infected by heterosexual transmission in Shanghai in 2013 was mainly CRF01_AE and CRF07_BC, but CRF07_BC subtype was predominant among those infected with heterosexual transmission and drug abuse history.