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作物叶片叶绿素含量与叶片光合能力密切相关,能影响农作物的产量。本研究通过EMS诱变甘蓝型油菜中双11,单株收获诱变后第一代并通过诱变后第二代大量筛选鉴定获得油菜叶片叶色稳定突变种质资源62份,分析了筛选获得的叶片叶色突变体的遗传分离比和测定了相关突变体的叶片叶绿素含量。结果显示,获得的突变体按照叶色分成五类,叶色深浅与其叶绿素含量相一致;突变株系60%由多位点突变导致,37%由少数位点突变导致;24%突变株系叶绿素含量明显高于对照,76%的叶绿素含量约低于对照;叶绿素含量低的突变株系中,70%的突变株系叶绿素b的改变程度大于叶绿素a,30%的突变株系叶绿素a的改变程度大于叶绿素b。这些叶色稳定突变体的获得为将来遗传基础研究以及作为种质资源培育高光效油菜新品种奠定材料基础。
Crop chlorophyll content is closely related to leaf photosynthetic capacity, can affect the crop yield. In this study, 62 pairs of Brassica napus were mutagenized by EMS and the first generation after single-strain mutagenesis was mutagenized, and 62 pieces of germplasm with stable leaf color were obtained by screening large-scale screening of the second generation after mutagenesis. The genetic segregation ratio of leaves leaf color mutants and the leaf chlorophyll content of the related mutants were determined. The results showed that the mutants were divided into five groups according to the leaf color, the leaf color depth was consistent with the chlorophyll content; 60% of the mutant lines were caused by multiple site mutations, 37% were caused by a few site mutations; 24% of the mutant lines were chlorophyll The content of chlorophyll b in 76% chlorophyll content was lower than that in the control. Among the mutants with low chlorophyll content, 70% of the mutants were more chlorophyll a than chlorophyll a, and 30% of the mutants were chlorophyll a To a greater extent than chlorophyll b. The availability of these leaf color stable mutants laid the material foundation for future basic genetic research and germplasm development for new varieties of high photosynthetic rape.