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许多实验研究证明,肺巨噬细胞不仅有很强的吞噬功能,而且参与某些免疫反应,因此被视为肺脏防御体系的重要组成部分,并引起人们的广泛重视。本文以家兔为实验材料,应用“洗肺”技术采取肺巨噬细胞,进行了超薄切片与冷冻蚀刻电镜观察,,以探讨巨噬细胞亚显微构造及其细胞膜结构与该细胞功能之间的关系。 (一)材料与方法:本实验用成年健康家兔,经放血处死后,立即切开气管并插入特制气管插管,注入适量(约50毫升)温生理盐水缓冲液,且随即吸出,如此反复灌洗6次。再将全部洗出液经三次离心沉淀(500克,8分钟/次),制得约5×10~6/毫升的肺巨噬细胞混悬液。接着,将离心后的细胞经4％戊二醛予固定,618树脂包埋,按常规方法制备超薄切片;同时,选用FHZ—1型冷冻蚀刻装置,制取冷冻蚀刻复型膜。最后送H—500透射
Many experimental studies have shown that pulmonary macrophages not only have strong phagocytic function, but also participate in certain immune responses, and therefore are considered as an important part of the lung defense system and aroused widespread attention. In this paper, rabbits as the experimental material, the application of “lung washing” technology to take pulmonary macrophages, ultra-thin sections and cryo-etching electron microscopy, to explore macrophages sub-microstructure and membrane structure and the cell function Relationship between. (A) Materials and Methods: In this experiment, adult healthy rabbits were sacrificed after exsanguination and immediately cut open the trachea and inserted into a special tracheal intubation, into the right amount (about 50 ml) warm saline buffer, and then sucked out, so repeated Lavage six times. The entire eluate was then centrifuged (500 g for 8 min) three times to produce a suspension of about 5 x 106 cells / ml of lung macrophages. Next, the centrifuged cells were fixed with 4% glutaraldehyde, embedded in 618 resin, and ultrathin sections were prepared according to a conventional method. At the same time, a freeze-etched reticle was prepared by using a FHZ-1 type freezing and etching apparatus. Finally send H-500 transmission