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目的:探讨活血利水之散血明目片对外伤性增生性玻璃体视网膜病变(traumatic Proliferrative Vitreo-retinopathy,tPVR)增殖膜(ERM)中整合素β1表达的影响及防治PVR的作用机理。方法:将40只成年有色家兔随机抽取32只,造成外伤性PVR模型,随机分成活血利水组、活血化瘀组、利水明目组、模型组,另8只为空白组。连续灌胃30天后,观察眼底PVR分级情况,免疫组织化学方法检测整合素β1表达在各组的表现及病理组织学改变。结果:在活血利水组,ERM中整合素β1阳性程度低于模型组,差异具有显著性(P<0.01)。活血化瘀组与利水明目组也能降低整合素β1表达的阳性程度,与模型组相比有显著性意义(P<0.05)。活血利水组整合素β1阳性程度低于另两个治疗组,有显著性差异(P<0.05)。活血利水治疗组整合素β1阳性程度略高于空白组,有显著差异性(P<0.01)。结论:活血利水法是活血化瘀和利水明目两者作用的协同,能通过拮抗增殖膜中整合素β1表达的作用来抑制增殖细胞的过度增生,从而防治PVR形成和发展。
Objective: To investigate the effect of “Sanxuemingshuetan Tablets” on integrin β1 expression in proliferative membrane (ERM) of traumatic Proliferrative Vitreo-retinopathy (tPVR) and its mechanism. Methods: Thirty-two adult pigmented rabbits were randomly selected and 32 rabbits were sacrificed. The traumatic PVR models were randomly divided into two groups: the Huoxueli group, the Huoxuehuayu group, the Liushui Mingmu group and the model group, and the other 8 as the blank group. After continuous gavage for 30 days, the classification of fundus PVR was observed. Immunohistochemistry was used to detect the expression of integrin β1 in each group and histopathological changes. Results: In the Huoxueli water group, the positive rate of integrin β1 in ERM was lower than that in the model group, the difference was significant (P <0.01). The blood-activating and stasis-removing groups and Lishui-Mingmu group also decreased the positive expression of integrin β1, which was significant compared with the model group (P <0.05). The level of integrin β1 in Huoxueli group was lower than that of the other two groups (P <0.05). Activating blood and water treatment group integrin β1 positive degree slightly higher than the blank group, there was a significant difference (P <0.01). CONCLUSION: The method of promoting blood circulation and promoting blood circulation is synergetic between the two functions of promoting blood circulation and removing blood stasis, and can prevent and treat the formation and development of PVR by inhibiting the proliferation of proliferating cells by antagonizing the expression of integrin β1 in proliferative membrane.