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目的:评估脊髓灰质炎(脊灰)实验室的监测状况及我省脊灰病毒(PV)核苷酸序列变异情况。方法:分析11个市急性弛缓性麻痹(AFP)病例监测系统经计算机联网上报的AFP病例个案调查表录入的数据库、国家及我省脊灰实验室监测的数据。结果:2006年AFP病例监测系统共报告AFP病例481例,省脊灰实验室共收集粪便标本950份,所有粪便标本用L20B(转人脊灰病毒受体的小鼠肺细胞系)、RD(人横纹肌肉瘤细胞系)同时进行肠道病毒(EV)分离。从31例AFP病例粪便标本中分离到PV,分离率为6.5%,并对所有PV进行了血清定型。从71例AFP病例粪便标本中分离到NPEV,分离率为14.9%。将PV混合株进行单型分离后,共计38株。经国家脊灰实验室对38株PV重新进行血清中和试验复核,结果符合率为100%。同时国家脊灰实验室还使用酶联免疫吸附试验(ELISA)和聚合酶链反应-限制性酶切片断长度多态性分析(PCR-RFLP)两种方法进行型内鉴定,并对其中27株进行了VP1编码区全基因的序列测定和分析,未检出疫苗衍生脊灰病毒(VDPV)或野毒株,全部为脊灰疫苗株。结论:河北省2006年继续维持无脊灰状态,脊灰实验室各项监测指标均达到世界卫生组织的要求,细胞系敏感性没有降低,脊灰实验室质量控制良好,继续为维持无脊灰提供了完整、可靠的实验室依据。
OBJECTIVE: To assess the monitoring status of the poliomyelitis (polio) laboratory and the variation of the nucleotide sequence of poliovirus (PV) in our province. Methods: The data of 11 cases of AFP cases monitoring system recorded by the computer-aided case-based questionnaires of AFP cases, the data of the countries and the polio laboratory in our province were analyzed. Results A total of 481 cases of AFP were reported by the AFP case surveillance system in 2006. A total of 950 stool samples were collected from the provincial polio laboratory. All stool specimens were stained with L20B (mouse lung cell line transfected with poliovirus receptor), RD Human rhabdomyosarcoma cell line) simultaneously with enterovirus (EV) isolation. PV was isolated from stool specimens of 31 AFP cases with a resolution of 6.5% and all PVs were serotyped. NPEV was isolated from 71 cases of AFP stool samples and the isolation rate was 14.9%. After the PV hybrids were subjected to single-type isolation, a total of 38 strains were obtained. The national polio laboratory 38 PV re-serum neutralization test review, the results of 100% compliance. At the same time, the national polio laboratory also used the enzyme-linked immunosorbent assay (ELISA) and the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) two methods for in-vivo identification, and of which 27 The whole genome of VP1 coding region was sequenced and analyzed. No vaccine-derived poliovirus (VDPV) or wild-type virus was detected, all of which were poliovirus strains. CONCLUSION: Hebei Province maintained its polio-free status in 2006. The monitoring indicators of the polio laboratory met the requirements of the World Health Organization. The cell line sensitivity did not decrease and the polio laboratory quality control was good. Provides a complete and reliable laboratory basis.