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最近在喀麦隆海岸地区发现2例氯喳抗性病例,因而对邻近地区进行了氯喹抗性株的流行情况调查。从西喀麦隆3个地区收集无近期服药史的5～15岁儿童的血液,肝素抗凝,冰冻带回实验室。共查了2429名儿童的血样,其中124名原虫血症阳性。弃去密度<0.1%者,得到76份分离物,于采样后5小时内进行培养,作下列两项实验:①48小时增殖试验;②26小时WHO微量试验。实验方法与标准法之不同为:不以推血片计数原虫密度为指
Two recent cases of chloroquine resistance were found in the coast of Cameroon and a survey of the prevalence of chloroquine resistant strains was carried out in neighboring areas. Blood from 5 to 15-year-old children without immediate medication history was collected from 3 western Cameroon anticoagulants and frozen back to the laboratory. A total of 2429 children were examined for blood samples, 124 of which were positive for parasitemia. Discard the density of <0.1% to get 76 isolates, cultured within 5 hours after sampling for the following two experiments: ① 48 hours proliferation test; ② 26 hours WHO micro test. The difference between the experimental method and the standard method is: do not count the protozoan density by pushing the blood chip as the reference