酶联免疫吸附试验用于检测和鉴别人疟媒介内的子孢子

• 作 者: 盛伯梁
• 出 处: 国外医学(寄生虫病分册)
• 发表日期: 1987年04期
• 关键词: 子孢子 海军医学研究所 现场鉴定 间日疟原虫 恶性疟原虫 恶性疟 间日疟 单克隆抗体 IgG 环子孢子蛋白

昆虫学家判定疟疾媒介历来以在其唾腺内发现子孢子为依据,此法操作烦琐且在现场鉴定的标本数量受到限制,更主要的是发现子孢子后仍难以根据形态鉴别疟原虫的虫种,其结果也不可靠。用酶联免疫吸附试验(ELISA)则可解决不少问题。1985年美国的纽约大学,Walter Reed陆军研究所及海军医学研究所合作,制成抗恶性疟原虫和间日疟原虫(子孢子)的环子孢子蛋白质的单克隆抗体(IgG)。其中的2A10与NMR1-3分别在抗恶性疟和间日疟子孢子时显示有较高的敏感性与特异性。目前用此法已能检测带有

Entomologists have determined that the malaria vector has historically been based on the discovery of sporozoites in its salivary glands. This method is cumbersome and the number of specimens identified in the field is limited. More importantly, it is still difficult to identify parasites of the parasite according to their morphology The result is not reliable. Enzyme-linked immunosorbent assay (ELISA) can solve many problems. In 1985, the New York University, Walter Reed Army Institute and the Institute of Naval Medical Research of the United States collaborated to create a monoclonal antibody (IgG) for circumsporozoite protein of Plasmodium falciparum and Plasmodium vivax (sporozoites). Among them, 2A10 and NMR1-3 showed higher sensitivity and specificity in the sporozoites of P. falciparum and P. vivax respectively. At present, this method has been tested with