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目的 研究维A胺酸质量控制方法。方法 用反相高效液相色谱法和正相高效液相色谱法。结果 反相色谱法中维A胺酸在 0 0 5～ 0 5 μg·μL-1与峰面积呈良好线性关系 ,检出限为 3ng ;正相色谱法中 ,维A酸的检出限为 5ng ;并用两种方法同时检测了 3批维A胺酸样品。 结论 维A胺酸及其异构体分离良好 ,样品中未检出维A酸和对氨基苯甲酸。反相色谱法简便、准确、重现性好 ,适用于维A胺酸及其杂质的质量控制 ;正相色谱法对维A胺酸、维A酸和对氨基苯甲酸分离较好 ,可用于控制维A胺酸中可能存在的原料维A酸和对氨基苯甲酸
Aim To study the quality control method of Vitamin A Methods Reversed-phase high performance liquid chromatography and normal-phase high performance liquid chromatography. Results Reversed-phase HPLC showed a good linear relationship between the content of retinoic acid and the peak area in the range of 0 0 5 ~ 0 5 μg · μL-1 with a detection limit of 3 ng. The detection limit of retinoic acid in normal phase chromatography was 5 ng; and three batches of Vitamin A acid samples were tested simultaneously by two methods. Conclusion Vitamin A and its isomers are well separated and no retinoids and p-aminobenzoic acid were detected in the samples. Reversed-phase chromatography is simple, accurate and reproducible. It is suitable for the quality control of Vitamin A and its impurities. Normal phase chromatography is better for the separation of Vitamin A, Vitamin A and p-aminobenzoic acid. Vitamin A acid and p-aminobenzoic acid may be the raw materials for the control of vitamin A acid